ATGGCCCAGTCCTCCTCCACCTCTCCAGACCAGGGAGCCACGTTCGAGCA
CCTGTGGAGCTCCCTGGAGCCAGACAGCACCTACTTTGACCTTCCCCAGC
CAAGTAGGGGGAATAATGAGGAAGTGGGCAGCGCAGAAGCCAGCATGGAT
GTGTTCCACCTGCAGGGCATGACCACATCTGTCATGGCCCAGTTCAATTT
GCTCAGCAGCACCATGGACCAGATGAGCAGCCGCGCGGCCTCGGCCAGCC
CCTACACCCCGGAGCACACTGCCAGCGTGCCCACCCACTCGCCCTACGCA
CAGCCCAGCTCCACCTTCGACACCATGTCGCCAGCGCCTGTCATCCCCTC
CAACACCGACTACCCTGGCCCCCACCACTTCGAGGTCACCTTCCAGCAGT
CTAGCACGGCCAAGTCTGCCACCTGGACGTACTCCCCGCTGTTGAAGAAG
CTGTACTGCCAAATCGCCAAGACGTGCCCCATCCAGATCAAGGTGTCCAC
CCCGCCACCCCCGGGCACCGCCATCCGGGCCATGCCTGTCTACAAGAAAG
CGGAGCATGTGACGGACATTGTGAAGCGCTGCCCGAACCATGAGCTTGGG
AGGGACTTCAACGAAGGACAGTCTGCCCCAGCCAGCCACCTCATCCGCGT
GGAGGGCAACAACCTCTCCCAGTATGTGGACGACCCCGTCACCGGCAGGC
AGAGCGTTGTGGTGCCCTATGAGCCCCCACAGGTAGGAACAGAATTCACC
ACCATCCTATACAACTTCATGTGTAACAGCAGCTGTGTGGGAGGCATGAA
TCGGCGGCCCATCCTCATCATCATCACCCTGGAGACGCGGGATGGACAGG
TCCTGGGCCGCCGGTCCTTTGAGGGACGTATCTGTGCCTGTCCTGGCCGT
GACCGCAAAGCTGACGAGGACCACTATCGAGAGCAGCAAGCCCTGAATGA
GAGCACTGCAAAGAATGGGTCCACCAGCAAACGTGCATTCAAGCAGAGCC
CCCCTGCCATCCCTGCCCTGGGTGCCAGTGTGAAGAAGAGGCGGCATGGG
GACGAGGACACATACTACATGCATGTGCGAGGCCGGGAGAACTTCGAGAT
CCTAATGAAGGTCAAGGAGAGCCTGGAGCTGATGGAACTGGTGCCACAGC
CACTGGTCGACTCCTACCGGCAGCAGCAGCAGCTCCTGCAGAGGCCGCCC
CGGGATGCTCAACAACCACAGCCACACTGTGCCCACTAATGGCGAGATGA
ACGGTGGCCATGGCTCCCAGCCCATGGTCTCGGGGTCCCACTGCACTCCA
CCACCCCCCTACCATGCTGACCCCAGCCTCGTCAGGATCTCGGGGCCCTG
AAGATCCCTGACCAGTACCGCATGACCATCTGGCGGGGCCTGCAGGATCT
GAAGCAGGGCCATGACTACGGCCCCCAGCAACTGCTTCGTTCCAGCAGCA
ATGCTGCTACCATCTCCATTGGCAGCTCGGGGGAGCTGCAGCGGCAGCGG
GTCATGGAGGCCGTGCACTTCCGCGTGCGCCACACCATCACCATCCCCAA
CCGTGGAGGTGGCCCAGGTGGTGGCACAGGCCCTGATGAGTGGGCAGACT
TTGGCTTCGACTTGCCCGACTGCAAGGCTCGCAAACAGCCCATCAAGGAG
GAGTTCACAGAGAGCGAGATCCAC

 Instructions:
 *** -> premature termination codon;  # -> frame shift 

 Alignment:
 Human     pep sequence --->
 ||||||||||||||||||||||
 Treeshrew pep sequence --->
 Treeshrew cds sequence --->

MetAlaGlnSerThrAlaThrSerProAspGlyGlyThrThrPheGluHisLeuTrpSer
||||||||||||      ||||||||||||   |||   |||||||||||||||||||||
MetAlaGlnSerSerSerThrSerProAspGlnGlyAlaThrPheGluHisLeuTrpSer
ATGGCCCAGTCCTCCTCCACCTCTCCAGACCAGGGAGCCACGTTCGAGCACCTGTGGAGC

Ser{Le}  >>>> Target Intron 1 >>>>  {u}GluProAspSerThrTyrPhe
|||{||}            812 bp           {|}|||||||||||||||||||||
Ser{Le}++                         ++{u}GluProAspSerThrTyrPhe
TCC{CT}gt                         ag{G}GAGCCAGACAGCACCTACTTT

AspLeuProGlnSerSerArgGlyAsnAsnGluValValGlyGlyThrAspSerSerMet
||||||||||||   ||||||||||||||||||   ||||||            ||||||
AspLeuProGlnProSerArgGlyAsnAsnGluGluValGlySerAlaGluAlaSerMet
GACCTTCCCCAGCCAAGTAGGGGGAATAATGAGGAAGTGGGCAGCGCAGAAGCCAGCATG

AspValPheHisLeuGluGlyMetThrThrSerValMet  >>>> Target Intron
|||||||||||||||   |||||||||||||||||||||           25153 bp
AspValPheHisLeuGlnGlyMetThrThrSerValMet++
GATGTGTTCCACCTGCAGGGCATGACCACATCTGTCATGgt

2 >>>>  AlaGlnPheAsnLeuLeuSerSerThrMetAspGlnMetSerSerArgAlaA
        ||||||||||||||||||||||||||||||||||||||||||||||||||||
      ++AlaGlnPheAsnLeuLeuSerSerThrMetAspGlnMetSerSerArgAlaA
      agGCCCAGTTCAATTTGCTCAGCAGCACCATGGACCAGATGAGCAGCCGCGCGG

laSerAlaSerProTyrThrProGluHisAlaAlaSerValProThrHisSerProTyrA
|||||||||||||||||||||||||||||   ||||||||||||||||||||||||||||
laSerAlaSerProTyrThrProGluHisThrAlaSerValProThrHisSerProTyrA
CCTCGGCCAGCCCCTACACCCCGGAGCACACTGCCAGCGTGCCCACCCACTCGCCCTACG

laGlnProSerSerThrPheAspThrMetSerProAlaProValIleProSerAsnThrA
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
laGlnProSerSerThrPheAspThrMetSerProAlaProValIleProSerAsnThrA
CACAGCCCAGCTCCACCTTCGACACCATGTCGCCAGCGCCTGTCATCCCCTCCAACACCG

spTyrProGlyProHisHisPheGluValThrPheGlnGlnSerSerThrAlaLysSerA
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
spTyrProGlyProHisHisPheGluValThrPheGlnGlnSerSerThrAlaLysSerA
ACTACCCTGGCCCCCACCACTTCGAGGTCACCTTCCAGCAGTCTAGCACGGCCAAGTCTG

laThrTrpThr  >>>> Target Intron 3 >>>>  TyrSerProLeuLeuLysLy
|||||||||||           15403 bp          ||||||||||||||||||||
laThrTrpThr++                         ++TyrSerProLeuLeuLysLy
CCACCTGGACGgt                         agTACTCCCCGCTGTTGAAGAA

sLeuTyrCysGlnIleAlaLysThrCysProIleGlnIleLysValSerThrProProPr
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
sLeuTyrCysGlnIleAlaLysThrCysProIleGlnIleLysValSerThrProProPr
GCTGTACTGCCAAATCGCCAAGACGTGCCCCATCCAGATCAAGGTGTCCACCCCGCCACC

oProGlyThrAlaIleArgAlaMetProValTyrLysLysAlaGluHisValThrAspVa
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
oProGlyThrAlaIleArgAlaMetProValTyrLysLysAlaGluHisValThrAspIl
CCCGGGCACCGCCATCCGGGCCATGCCTGTCTACAAGAAAGCGGAGCATGTGACGGACAT

lValLysArgCysProAsnHisGluLeuGlyArgAspPheAsnGlu{G}  >>>> Targ
 |||||||||||||||||||||||||||||||||||||||||||||{|}
eValLysArgCysProAsnHisGluLeuGlyArgAspPheAsnGlu{G}++
TGTGAAGCGCTGCCCGAACCATGAGCTTGGGAGGGACTTCAACGAA{G}gt

et Intron 4 >>>>  {ly}GlnSerAlaProAlaSerHisLeuIleArgValGluGl
 466 bp           {||}||||||||||||||||||||||||||||||||||||||
                ++{ly}GlnSerAlaProAlaSerHisLeuIleArgValGluGl
                ag{GA}CAGTCTGCCCCAGCCAGCCACCTCATCCGCGTGGAGGG

yAsnAsnLeuSerGlnTyrValAspAspProValThrGlyArgGlnSerValValValPr
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
yAsnAsnLeuSerGlnTyrValAspAspProValThrGlyArgGlnSerValValValPr
CAACAACCTCTCCCAGTATGTGGACGACCCCGTCACCGGCAGGCAGAGCGTTGTGGTGCC

oTyrGluProProGln  >>>> Target Intron 5 >>>>  ValGlyThrGluPhe
||||||||||||||||           1928 bp           |||||||||||||||
oTyrGluProProGln++                         ++ValGlyThrGluPhe
CTATGAGCCCCCACAGgt                         agGTAGGAACAGAATTC

ThrThrIleLeuTyrAsnPheMetCysAsnSerSerCysValGlyGlyMetAsnArgArg
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ThrThrIleLeuTyrAsnPheMetCysAsnSerSerCysValGlyGlyMetAsnArgArg
ACCACCATCCTATACAACTTCATGTGTAACAGCAGCTGTGTGGGAGGCATGAATCGGCGG

ProIleLeuIleIleIleThrLeuGluMetArg{As}  >>>> Target Intron 6
|||||||||||||||||||||||||||   |||{||}            434 bp
ProIleLeuIleIleIleThrLeuGluThrArg{As}++
CCCATCCTCATCATCATCACCCTGGAGACGCGG{GA}gt

>>>>  {p}GlyGlnValLeuGlyArgArgSerPheGluGlyArgIleCysAlaCysPro
      {|}|||||||||||||||||||||||||||||||||||||||||||||||||||
    ++{p}GlyGlnValLeuGlyArgArgSerPheGluGlyArgIleCysAlaCysPro
    ag{T}GGACAGGTCCTGGGCCGCCGGTCCTTTGAGGGACGTATCTGTGCCTGTCCT

GlyArgAspArgLysAlaAspGluAspHisTyrArgGluGlnGlnAlaLeuAsnGluSer
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GlyArgAspArgLysAlaAspGluAspHisTyrArgGluGlnGlnAlaLeuAsnGluSer
GGCCGTGACCGCAAAGCTGACGAGGACCACTATCGAGAGCAGCAAGCCCTGAATGAGAGC

SerAlaLysAsnGlyAlaAlaSerLysArg{A}  >>>> Target Intron 7 >>>>
   ||||||||||||      |||||||||{|}            447 bp
ThrAlaLysAsnGlySerThrSerLysArg{A}++
ACTGCAAAGAATGGGTCCACCAGCAAACGT{G}gt

  {la}PheLysGlnSerProProAlaValProAlaLeuGlyAlaGlyValLysLysArg
  {||}|||||||||||||||||||||   |||||||||||||||   ||||||||||||
++{la}PheLysGlnSerProProAlaIleProAlaLeuGlyAlaSerValLysLysArg
ag{CA}TTCAAGCAGAGCCCCCCTGCCATCCCTGCCCTGGGTGCCAGTGTGAAGAAGAGG

ArgHisGlyAspGluAspThrTyrTyrLeuGln  >>>> Target Intron 8 >>>>
|||||||||||||||||||||||||||                  843 bp
ArgHisGlyAspGluAspThrTyrTyrMetHis++
CGGCATGGGGACGAGGACACATACTACATGCATgt

  ValArgGlyArgGluAsnPheGluIleLeuMetLysLeuLysGluSerLeuGluLeuM
  ||||||||||||||||||||||||||||||||||||   |||||||||||||||||||
++ValArgGlyArgGluAsnPheGluIleLeuMetLysValLysGluSerLeuGluLeuM
agGTGCGAGGCCGGGAGAACTTCGAGATCCTAATGAAGGTCAAGGAGAGCCTGGAGCTGA

etGluLeuValProGlnProLeuValAspSerTyrArgGln  >>>> Target Intro
|||||||||||||||||||||||||||||||||||||||||            341 bp
etGluLeuValProGlnProLeuValAspSerTyrArgGln+-
TGGAACTGGTGCCACAGCCACTGGTCGACTCCTACCGGCAGga

n 9 >>>>  GlnGlnGlnLeuLeuGlnArg{Pr}  >>>> Target Intron 10 >
          |||||||||||||||||||||{||}            1652 bp
        ++GlnGlnGlnLeuLeuGlnArg{Pr}++
        agCAGCAGCAGCTCCTGCAGAGG{CC}gt

>>>  {o}ProArgAspAlaGlnGlnProTrpProArgSerAlaSerGlnArgArgAspG
     {|}|||||||||||||||||||||   |||      |||         |||||||
   ++{o}ProArgAspAlaGlnGlnProGlnProHisCysAlaHis***TrpArgAspG
   ag{G}CCCCGGGATGCTCAACAACCACAGCCACACTGTGCCCACTAATGGCGAGATG

luGlnGlnProGlnArgProValHisGlyLeuGlyValProLeuHisSerAlaThrProL
||      |||      |||   |||||||||||||||||||||||||||   |||||||
luArgTrpProTrpLeuProAlaHisGlyLeuGlyValProLeuHisSerThrThrProL
AACGGTGGCCATGGCTCCCAGCCCATGGTCTCGGGGTCCCACTGCACTCCACCACCCCCC

euProArgArgProGlnProArgGln  >>>> Target Intron 11 >>>>  AspL
|||||      |||||||||||||||            2001 bp           ||||
euProCys***ProGlnProArgGln++                          ++AspL
TACCATGCTGACCCCAGCCTCGTCAGgt                          agGATC

euGlyAlaLeuLysIleProGluGlnTyrArgMetThrIleTrpArgGlyLeuGlnAspL
||||||||||||||||||||   |||||||||||||||||||||||||||||||||||||
euGlyAlaLeuLysIleProAspGlnTyrArgMetThrIleTrpArgGlyLeuGlnAspL
TCGGGGCCCTGAAGATCCCTGACCAGTACCGCATGACCATCTGGCGGGGCCTGCAGGATC

euLysGlnGlyHisAspTyrSerThrAlaGlnGlnLeuLeuArgSerSerAsnAlaAlaT
||||||||||||||||||||         |||   |||      ||||||||||||||||
euLysGlnGlyHisAspTyrGlyProGlnGlnLeuLeuArgSerSerSerAsnAlaAlaT
TGAAGCAGGGCCATGACTACGGCCCCCAGCAACTGCTTCGTTCCAGCAGCAATGCTGCTA

hrIleSerIleGlyGlySerGlyGluLeuGlnArgGlnArgValMetGluAlaValHisP
||||||||||||||   |||||||||||||||||||||||||||||||||||||||||||
hrIleSerIleGlySerSerGlyGluLeuGlnArgGlnArgValMetGluAlaValHisP
CCATCTCCATTGGCAGCTCGGGGGAGCTGCAGCGGCAGCGGGTCATGGAGGCCGTGCACT

heArgValArgHisThrIleThrIleProAsnArg<->GlyGlyProGlyGly<-><->G
|||||||||||||||||||||||||||||||||||   |||||||||||||||      |
heArgValArgHisThrIleThrIleProAsnArgGlyGlyGlyProGlyGlyGlyThrG
TCCGCGTGCGCCACACCATCACCATCCCCAACCGTGGAGGTGGCCCAGGTGGTGGCACAG

lyProAspGluTrpAlaAspPheGlyPheAspLeuProAspCysLysAlaArgLysGlnP
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
lyProAspGluTrpAlaAspPheGlyPheAspLeuProAspCysLysAlaArgLysGlnP
GCCCTGATGAGTGGGCAGACTTTGGCTTCGACTTGCCCGACTGCAAGGCTCGCAAACAGC

roIleLysGluGluPheThrGluAlaGluIleHis
|||||||||||||||||||||||   |||||||||
roIleLysGluGluPheThrGluSerGluIleHis
CCATCAAGGAGGAGTTCACAGAGAGCGAGATCCAC


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