Instructions:
*** -> premature termination codon; # -> frame shift
Alignment:
Human pep sequence --->
||||||||||||||||||||||
Treeshrew pep sequence --->
Treeshrew cds sequence --->
AlaLeuAlaValPheThrGlnLeuProGlnAspThrSerValGluValGlyLysAsnI
||| ||| |||||||||||| ||| ||||||
AlaMetProSerPheValIleArgProGlnAspThrGluValLeuValGlyThrSerT
GCCATGCCCAGCTTTGTCATCCGGCCCCAGGACACAGAGGTGCTGGTTGGCACCAGCA
leAsnIleSerCysHisAlaGlnGlyGluProGlnProIleIleThrTrpAsnLys
||| ||| ||| ||| ||| ||||||
hrThrLeuGluCysValAlaThrGlyHisProLeuProHisValThrTrpThrArg+-
CGACCCTGGAGTGCGTGGCCACAGGCCACCCGCTCCCCCACGTCACTTGGACCAGGgg
>>>> Target Intron 1 >>>> GluGlyValGlnIleThrGluSerGlyLysP
36638 bp ||||||||||||||||||||||||||||
++AlaGlyValGlnIleThrGluSerGlyLysP
agGCAGGTGTGCAGATTACGGAGAGCGGTAAGT
heHisValAspAspGluGlyThrLeuThrIleTyrAspAlaGlyPheProAspGlnGl
|| ||| ||||||||||||||||||||||||||| ||||||||
heArgMetAspGly---GlyThrLeuThrIleTyrAspAlaGlyHisAlaAspGlnGl
TCCGCATGGACGGA---GGCACGCTGACCATCTATGACGCGGGCCATGCGGACCAAGG
yArgTyrGluCysValAlaArgAsnSerPheGlyLeuAlaValThrAsnMetPheLeu
| ||| ||||||||||||||| ||||||||| ||| ||||||
yGlnTyrAspCysValAlaArgAsnProPheGlyLeuValValAlaThrAlaPheLeu
ACAATACGACTGCGTGGCTCGGAACCCCTTCGGCCTGGTGGTGGCCACCGCATTCCTC
ThrValThr{A} >>>> Target Intron 2 >>>> {la}IleGlnGlyArgG
|||||||||{|} 18269 bp {||} |||||||||
ThrValThr{A}++ ++{la}AlaGlnGlyArgA
ACCGTCACC{G}gt ag{CA}GCACAGGGTAGAC
lnAlaGlyAspAspPheValGluSerSerIleLeuAspAlaValGlnArgValAspSe
||||||||| |||||||||||||||||||||||||||||| |||||||||||
rgAlaGlyAspAlaPheValGluSerSerIleLeuAspAlaValArgArgValAspSe
GAGCCGGTGACGCCTTCGTGGAATCATCCATCCTTGATGCTGTGCGGAGGGTTGACAG
rAlaIleAsnSerThrArgArgHisLeuPheSer{Gl} >>>> Target Intron
||||||||||||||||||||||||||||||||||{||} 1232 bp
rAlaIleAsnSerThrArgArgHisLeuPheSer{Gl}++
TGCAATTAACTCCACACGACGACACTTGTTTTCA{CA}gt
3 >>>> {n}LysProHisThrSerSerAspLeuLeuAlaGlnPheHisTyrProA
{|} |||||||||||| |||||||||||| |||||||||||||
++{n}ArgProHisThrSerGlyAspLeuLeuAlaLeuPheHisTyrProA
ag{G}AGACCGCACACCTCCGGGGATCTGCTGGCTCTCTTCCACTACCCAC
rgAspProLeuIleValGluMetAlaArgAlaGlyGluIlePheGluHisThrLeuGl
|||||||| ||| ||||||||||||||| ||| ||||||||
rgAspProPheThrValValValAlaArgAlaGlyGluValPheThrGlnThrLeuGl
GGGACCCGTTCACGGTGGTGGTGGCCAGAGCTGGGGAGGTCTTCACGCAGACACTGCA
nLeuIleArgGluArgValLysGlnGlyLeuThrValAspLeuGluGlyLys{G} >
|||||||||| ||||||||| |||||||||||| ||| {|}
nLeuIleArgAspHisValLysGlnAlaLeuThrValAspLysGlnGlyPro{G}++
GCTGATCCGGGACCACGTGAAGCAGGCGCTGACCGTGGACAAGCAGGGCCCA{G}gt
>>> Target Intron 4 >>>> {lu}PheArgTyrAsnAspLeuValSerProA
2629 bp {||}|||||||||||| |||||||||||||
++{lu}PheArgTyrAsnAlaLeuValSerProA
ag{AG}TTCCGCTACAACGCCTTGGTGTCCCCGC
rgSerLeuSerLeuIleAlaAsnLeuSerGlyCysThrAlaArgArgProLeuProAs
|||||||||||||| ||| |||||| |||||||||||||||||| ||
rgSerLeuSerLeuMetAlaAspValSerGlyTyrThrAlaArgArgProLeuLeuAs
GCTCCCTCAGCCTCATGGCCGACGTGTCAGGGTACACAGCCCGCCGGCCTCTGCTAAA
nCysSerAsnArgCysPheHisAlaLysTyrArgAlaHisAspGlyThrCysAsnAsn
||||||| ||| ||| |||||||||||| ||| |||||||||
nCysSerAspLeuCysLeuHisArgLysTyrArgAlaAlaAspArgAlaCysAsnAsn
CTGCTCCGACCTGTGTTTGCACCGCAAGTACCGAGCAGCGGACAGAGCCTGCAACAAC
LeuGlnGlnProThrTrpGlyAlaAlaLeuThrAlaPheAlaArgLeuLeuGlnProA
|||||| ||||||||||||||||||||| |||||||||||| ||||||||||
LeuGlnHisProThrTrpGlyAlaAlaLeuGlyAlaPheAlaArgIleLeuGlnProA
CTGCAGCACCCGACCTGGGGCGCGGCTCTGGGCGCCTTCGCGCGCATCCTGCAGCCGG
laTyrArgAspGlyIleArgAlaProArgGlyLeuGlyLeuProValGlySerArgGl
||||| ||| |||||| ||
laTyrLysAspAlaMetMetArgLeuAlaGlyLeuAlaAlaThrTrpThrGlnAlaGl
CCTACAAGGATGCTATGATGCGCCTCGCGGGGCTGGCCGCCACCTGGACGCAGGCCCA
n--ProLeuProProProArgLeuValAlaThrValTrpAlaArgAlaAlaAlaValT
|##||||||||| |||||||||||| |||
n##ProLeuProLeuProArgLeuValSerAlaGluLeuValGlyMetAlaGlyGlyA
GCGCCCCTCCCGCTGCCCAGGCTGGTCTCCGCGGAGCTGGTGGGCATGGCGGGCGGGG
hrProAsp{H} >>>> Target Intron 5 >>>> {is}SerTyrThrArgMe
||| {|} 67 bp {||} ||| ||
laProPro{H}-- +-{is}ThrHisThrHisMe
CCCCCCCC{C}nn ac{AC}ACACACACACACAT
tLeuMetHisTrpGlyTrpPheLeuGluHisAspLeuAspHisThrValProAlaLeu
|||||||||||||||| |||||||||||||||||||||||| |||||||||
tLeuMetHisTrpGlyArgPheLeuGluHisAspLeuAspHisAlaLeuProAlaLeu
GCTCATGCACTGGGGCCGGTTTCTGGAGCACGACCTGGACCACGCCTTGCCGGCGCTG
SerThrAlaArgPheSerAspGlyArgProCysSerSerValCysThrAsnAspProP
|||||| ||||||||| |||||||||||| ||| |||||||
GlyThrAlaCysPheSerAspAlaLysProCysSerSerAlaCysSerHisAspProP
GGCACCGCGTGCTTCTCGGACGCAAAGCCCTGCAGCTCCGCCTGCTCGCACGACCCTC
roCysPheProMetAsnThrArgHisAlaAspProArgGlyThrHisAlaProCysMe
||||| ||| ||| |||||||||
roCysLeuProValAspTyrProArgThrAspSerTrpArgAlaGlnAlaProCysAr
CCTGCCTCCCTGTGGACTACCCTCGCACTGACTCCTGGAGGGCCCAAGCACCTTGCAG
tLeuPheAlaArgSerSerProAlaCysAlaSerGlyArgProSerAlaThrValAsp
|||||||||||| ||| |||
gThrLeuAlaArgSerSerSerValCysGlyIleAspLeuThrSerSerGlyArgAsn
AACGTTGGCACGCTCCAGCTCAGTGTGTGGCATCGACCTGACCTCTTCCGGAAGGAAC
SerValTyrAlaArgGluGlnIleAsnGlnGlnThrAlaTyrIleAspGlySerAsnV
|||||| ||||||||| |||||| ||||||||| ||| |||
SerValHisThrArgGluGlnMetAsnGlnProThrAlaTyrLeuAspAlaSerSerI
TCTGTCCATACCCGGGAGCAGATGAACCAGCCCACAGCCTACCTCGATGCCTCCAGCA
alTyrGlySerSerGluArgGluSerGlnAlaLeuArgAspProSerValProArgGl
|||||||||||| |||||| |||||| ||| ||
leTyrGlySerSerGlnTrpGluSerLeuMetPheArgAspHisAlaGluProTrpGl
TTTATGGGAGCTCGCAGTGGGAATCTCTGATGTTCAGAGACCATGCGGAGCCGTGGGG
yLeuLeuLysThrGlyPheProTrpProProSerGlyLysProLeuLeuProPheSer
||||||| ||| ||| ||||||||||||||| |||
yLeuLeuArgThrSerSerArgTrpAlaProSerGlyLysPro---SerProAlaPro
ACTGCTGAGGACCAGCTCGCGGTGGGCCCCTTCTGGAAAGCCC---TCCCCGGCGCCC
ThrGlyProProThrGluCysAlaArgGlnGluGlnGluSerProCysPheLeuAlaG
||| |||||||||||||
ProAlaThrGly***AlaGlyProGlyGlnAlaArgArgGlyArgCysPheLeuAlaG
CCAGCCACTGGGTGAGCAGGCCCTGGCCAGGCCAGACGCGGGCGCTGCTTCCTGGCAG
lyAspHisArgAlaAsnGluHisLeuAlaLeuAlaAlaMetHisThrLeuTrpPheAr
||||| |||||||||||| |||||| ||||||||||||||||||
lyAspTyrArgAlaAsnGluGlnProAlaLeuLeuAlaMetHisThrLeuTrpLeuGl
GGGACTACCGCGCCAACGAGCAGCCGGCTCTGCTGGCCATGCACACCCTGTGGCTCGG
gGluHisAsnArgMetAlaThrGluLeuSerAlaLeuAsnProHisTrpGluGlyAsn
||||||||| ||| ||||||||| ||||||||||||||| |||
yAspHisAsnArgValAlaSerGluLeuSerThrLeuAsnProHisTrpAspGlyAsp
CGACCACAACAGGGTGGCCTCCGAGCTGTCGACCCTGAACCCCCACTGGGATGGGGAC
ThrValTyrGlnGluAlaArgLysIleValGlyAlaGluLeuGlnHisIleThrTyrS
||| |||||||||||||||||| |||||| |||||||||||||||||||
ThrLeuTyrGlnGluAlaArgLysValValGlyThrGlnLeuGlnHisIleThrTyrS
ACCCTCTACCAGGAGGCCAGGAAGGTCGTGGGCACGCAGCTGCAGCACATCACCTACA
erHisTrpLeuProLysValLeuGlyAspProGlyThrArgMetLeuArgGlyTyrAr
|||||||||||||||||||||||||| |||||| ||| |||
erHisTrpLeuProLysValLeuGlyGluProGlyMetLysValLeuGlyGluTyrGl
GCCACTGGCTGCCCAAGGTCCTGGGGGAGCCCGGCATGAAGGTGCTGGGTGAGTACCA
gGly--TyrAsnProAsnValAsnAlaGlyIleIleAsnSerPheAlaThrAlaAlaP
|||## ||| |||||||||||||||||| ||||||||| ||||||
nGly##---AspProThrValAsnAlaGlyIleIleIleSerPheAlaAlaAlaAlaS
GGGCTA---GACCCCACCGTGAACGCCGGCATCATCATCTCCTTCGCCGCTGCAGCCT
heArgPheGlyHisThrLeuIleAsnProIleLeuTyrArgLeuAsnAlaThrLeuGl
||| ||| |||||| ||||||||||||||| |||
erAlaLeuGlyArgAlaLeuThrAsnProValLeuTyrArgLeuAsnAspThrPheCy
CTGCACTGGGCCGCGCGTTAACTAATCCCGTCCTGTATCGCCTGAATGACACTTTCTG
yGluIleSerGluGlyHisLeuProPheHisLysAlaLeuPheSerProSerArgIle
|||||| ||||||||||||||| |||||||||||||||||| ||||||
sGluIleProGluGlyHisLeuProLeuHisLysAlaLeuPheSerSerSerArgLeu
TGAAATTCCCGAAGGCCACCTTCCGCTCCACAAAGCTCTCTTTTCTTCGTCCAGGCTA
IleLysGluGlyGlyIleAspProValLeuArgGlyLeuPheGlyValAlaAlaLysT
||| ||||||||||||||| ||||||||||||||||||||| |||
IleAspGluGlyGlyIleAspLeuLeuLeuArgGlyLeuPheGlyValGlyAlaGluG
ATAGACGAGGGTGGGATCGATCTGCTCCTCCGAGGGCTGTTCGGGGTGGGGGCTGAGC
rpArgAlaProSer-TyrLeuLeuSerProGluLeuThrGlnArgLeuPheSerAlaA
||| # ||| ||| ||| ||| |||||||
lnArgArgAlaAla#---LeuProThrProGlyLeuAlaArgSerLeuAlaSerAlaA
AGCGGCGGGCAGCGC---CTCCCAACCCCAGGCCTAGCTCGAAGCCTGGCCTCTGCTG
laTyrSerAlaAlaValAspSerAlaAlaThrIleIleGlnArgGlyArgAspHisGl
|| ||||||||| |||||||||||| |||||||||||||||||||||||
laArgSerAlaAlaHisAspSerAlaAlaValHisIleGlnArgGlyArgAspHisGl
CCCGTTCTGCCGCCCATGACTCAGCCGCTGTCCACATCCAAAGGGGTCGCGACCATGG
yIleProProTyrValAspPheArgValPheCysAsnLeuThrSerValLysAsnPhe
|||| |||||||||||||||||||||||||||||||||||| ||| ||||||
yIleLeuProTyrValAspPheArgValPheCysAsnLeuThrPheValGluAsnPhe
GATCCTGCCCTATGTCGACTTCAGGGTTTTCTGTAATTTGACTTTTGTTGAGAACTTT
GluAspLeuGlnAsnGluIleLysAspSerGluIleArgGlnLysLeuArg{Ly} >
|||||||||||||||||| ||||||||||||||||||||||||||| {||}
GluAspLeuGlnAsnGluMetLysAspSerGluIleArgGlnLysLeuLys{Ly}++
GAGGATCTTCAAAATGAAATGAAAGATTCAGAGATCAGACAAAAGCTGAAA{AA}gt
>>> Target Intron 6 >>>> {s}LeuTyrGlySerProGlyAspIleAspLe
45080 bp {|}|||||| ||| |||||||||||
++{s}LeuTyrThrThrProValAspIleAspLe
ag{G}TTGTACACCACGCCAGTGGACATCGACCT
uTrpProAlaLeuMetValGluAspLeuIleProGlyThrArgValGlyProThrLeu
||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
uTrpProAlaLeuLeuValGluAspLeuIleProGlyThrArgValGlyProThrLeu
CTGGCCTGCTCTTTTGGTGGAAGACCTGATCCCTGGCACCAGAGTGGGACCAACACTT
MetCysLeuPheValThrGlnPheGlnArgLeuArgAspGlyAsp{Ar} >>>> Ta
||||||||| |||||||||||| |||||||||||| {||}
MetCysLeuTyrValThrGlnPheArgArgLeuArgAspTyrLys{Ar}-+
ATGTGCCTGTATGTCACCCAGTTCCGGCGGCTGAGAGATTACAAA{AG}at
rget Intron 7 >>>> {g}PheTrpTyrGluAsnProGlyValPheThrProAl
5164 bp {|}|||||||||||||||||| ||||||||||||||
++{g}PheTrpTyrGluAsnProIleValPheThrProAl
ag{G}TTCTGGTATGAAAATCCCATAGTGTTCACCCCTGC
aGlnLeuThrGlnLeuLysGlnAlaSerLeuSerArgValLeuCysAspAsnGlyAsp
|||||||||||||||||||||| |||||| ||||||||||||||||||||||||
aGlnLeuThrGlnLeuLysGlnValSerLeuGlyArgValLeuCysAspAsnGlyAsp
TCAACTCACTCAGCTGAAACAGGTGTCCCTGGGCCGTGTGCTGTGTGACAATGGTGAC
SerIleGlnGlnValGlnAlaAspValPheValLysAlaGluTyrProGlnAspTyrL
||||||||||||||||||||||||||| |||||||||||||||||||||||||
AsnIleGlnGlnValGlnAlaAspValPheIleLysAlaGluTyrProGlnAspTyrL
AATATCCAGCAAGTCCAGGCTGATGTCTTCATAAAGGCGGAATACCCCCAGGACTACC
euAsnCysSerGluIleProLysValAspLeuArgValTrpGlnAspCysCysAla{A
|| |||||| ||||||||| ||||||||||||||||||||||||||| {
euSerCysSerValIleProLysIleAspLeuArgValTrpGlnAspCysCysGly{A
TGAGCTGCTCTGTGATCCCCAAGATCGATTTGCGAGTATGGCAAGACTGCTGCGGA{A
} >>>> Target Intron 8 >>>> {sp}CysArgSerArgGlyGlnPheArg
} 23503 bp { }||||||||||||
}++ ++{sn}CysArgSerArgGluArgLeuAla
}gt ag{AC}TGCAGGAGTCGAGAACGGCTCGCA
AlaValThrGlnGluSerGlnLysLysArgSerAlaGlnTyrSerTyrProValAspL
||| ||||||||||||||| |
GlyPheAlaAlaAspThrArgGlnProArgTrpAlaGlnTyrSerTyrGlyAspLysL
GGCTTTGCTGCAGACACCCGACAGCCCCGCTGGGCTCAGTATAGCTATGGGGACAAGA
ysAspMetGluLeuSerHisLeuArgSerArg
|| |||||| ||||||
ysAlaLysAspProGlyHisLeuGlySerArg
AAGCCAAGGATCCAGGCCACCTAGGCAGTAGG
//
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